The Glover River is the only remaining unimpounded tributary of the Little River. This highly pristine river is 48 km long, has a drainage area of 876 km2, and is known for its scenic value and fish biodiversity. The river contains the largest known population of the federally threatened leopard darter, Percina pantherina.

The upper two thirds of the river are high gradient (average slope 19 m/km) and characterized by low bedrock and boulder falls, chutes and riffles. Below the fall line, the gradient decreases to 1 m/km as the river flows through the Gulf Coastal Plain before entering the Little River. Here stream habitat is characterized by deep, long pools separated by shallow riffles. Riparian areas are mixed pine and oak forest historically harvested for timber by private companies. Since 1996, much of the watershed has come under the management of the U.S. Forest Service and timber harvest has decreased.